ChloroquineV1, Main, Exploration, bibRecord, 003645

A quantitative metabolic inhibition test for screening toxic compounds with cultured cells

Identifieur interne : 003645 ( Main/Exploration ); précédent : 003644; suivant : 003646

A quantitative metabolic inhibition test for screening toxic compounds with cultured cells

Auteurs : D. G. Wenzel [États-Unis] ; G. N. Cosma [États-Unis]

Source :

Toxicology [ 0300-483X ] ; 1983.

RBID : ISTEX:067581B9A408346BA6AA22541B6E3F5B3F76699B

Descripteurs français

KwdFr :
- Animaux, Cellules cultivées, Concentration en ions d'hydrogène, Fibroblastes (), Fibroblastes (métabolisme), Poumon (), Rats, Toxicologie, Évaluation préclinique de médicament ().
MESH :
- métabolisme : Fibroblastes.
Pascal (Inist)
- Animal, Animaux, Appareil respiratoire, Cellules cultivées, Concentration en ions d'hydrogène, Culture monocouche, Fibroblaste, Fibroblastes, Méthode étude, Poumon, Rat, Rats, Test inhibition métabolisme, Toxicité, Toxicologie, Évaluation préclinique de médicament.
Wicri :
- topic : Toxicologie.

English descriptors

KwdEn :
- Animal, Animals, Cells, Cultured, Drug Evaluation, Preclinical (methods), Fibroblast, Fibroblasts (drug effects), Fibroblasts (metabolism), Hydrogen-Ion Concentration, Investigation method, Lung, Lung (drug effects), Metabolism inhibition test, Monolayer culture, Rat, Rats, Respiratory system, Toxicity, Toxicology.
MESH :
- drug effects : Fibroblasts, Lung.
- metabolism : Fibroblasts.
- methods : Drug Evaluation, Preclinical.
- Animals, Cells, Cultured, Hydrogen-Ion Concentration, Rats, Toxicology.
Teeft :
- Anaerobic environment, Bacto trypsin, Chloroquine, Color change, Color changes, Color standards, Culture dishes, Culture medium, Cultured cells, Difco trypsin, Dioxide, Drug pairs, Drug toxicity, Ekwall, Hela, Hela cells, Inhibition test, Initial period, Lung fibroblasts, Metabolic inhibition test, Preliminary studies, Publishers ireland, Qmit, Qmit color grades, Qmit grades, Second subculture, Silicon, Silicon dioxide, Subculture, Test agent, Test agents, Toxicity, Toxicology, Uniform results.

Abstract

Abstract: The toxicity of substances for monolayer cultures of rat lung fibroblasts with phenol red as a pH indicator in the medium was measured by comparing the changes produced in medium color against 10 stable color standards. The toxicities of 6 concentrations each of actinomycin D, amphotericin B, chloroquine, 2-deoxyglucose, oligomycin, puromycin, and silicon dioxide were measured at 12 h and daily for 7 days on the same cultures throughout. Morphologic changes were monitored at the same times. The method, which simultaneously measures both concentration and time effects, was rapid, simple-to-perform, reproducible, low-in-cost, and quite sensitive. Its reproducibility depended on details of the cell culture methodology. The method is unsuitable for acidic or basic substances, or substances poorly-soluble in culture medium. Unless combined with morphologic evaluation, substances producing delayed toxicity may give misleading results.

Url:

https://api.istex.fr/ark:/67375/6H6-TPW44BLH-F/fulltext.pdf

DOI: 10.1016/0300-483X(83)90049-5

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The toxicity of substances for monolayer cultures of rat lung fibroblasts with phenol red as a pH indicator in the medium was measured by comparing the changes produced in medium color against 10 stable color standards. The toxicities of 6 concentrations each of actinomycin D, amphotericin B, chloroquine, 2-deoxyglucose, oligomycin, puromycin, and silicon dioxide were measured at 12 h and daily for 7 days on the same cultures throughout. Morphologic changes were monitored at the same times. The method, which simultaneously measures both concentration and time effects, was rapid, simple-to-perform, reproducible, low-in-cost, and quite sensitive. Its reproducibility depended on details of the cell culture methodology. The method is unsuitable for acidic or basic substances, or substances poorly-soluble in culture medium. Unless combined with morphologic evaluation, substances producing delayed toxicity may give misleading results.</div>
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Serveur d'exploration Chloroquine

A quantitative metabolic inhibition test for screening toxic compounds with cultured cells

A quantitative metabolic inhibition test for screening toxic compounds with cultured cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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